FastQCFastQC Report
Sat 4 Mar 2017
G94-M5_GGCTAC_seqtk.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG94-M5_GGCTAC_seqtk.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21445254
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG34450.013.2828393
CGTATGC37200.012.1255561
ATGCCGT40950.011.4428014
CCGCAAT12600.09.7793091
CCGGCAT31200.09.661771
GCCGTCT49650.09.6586756
CGCGAAT6050.09.4560271
ACGGCTA23150.09.40812332
TCGTATG25150.09.359364543
CTCGAAT25450.09.3374161
CCGTCTT52450.09.1849967
CCGGAAT24700.08.8191951
TGCCGTC55800.08.6735755
GTATGCC54500.08.5576822
CGCATAT7350.08.3822651
CGCGGAT9550.08.2944931
CGCAATT9100.08.2210681
CGCAAAT12850.08.2191871
CTCGTAT28400.08.1344742
CCGGGAT40100.08.0661011
Produced by FastQC (version 0.11.2)